

| 型號/Model | SPR-T200 | SPR-T210 | SPR-I300 | |
| 光源 Light Source |
類型/Type | He-Ne Laser | He-Ne Laser | He-Ne Laser,and LED |
| 波長/wavelength | 632.8nm | 632.8nm | 632.8nm,and 621±10nm | |
| 轉臺 Rotation Stage |
轉臺數量 Number of Rotation stage |
Motor 1, sample motor Motor 2, detector motor |
Motor 1, sample motor Motor 2, detector motor |
Motor 1, sample motor Motor 2, detector motor |
| 轉臺控制 Control of rotation stage |
軟件自動控制 Automatically control |
軟件自動控制 Automatically control |
軟件自動控制 Automatically control |
|
| 轉臺角度精度 Rotation angular resolution |
<= 0.02° 可升級/upgradable |
0.01° 可升級/upgradable |
0.01° 可升級/upgradable |
|
| 轉臺旋轉角度范圍 Rotation angles |
0-360°(Motor 1) 0-180°(Motor 2) |
0-360°(Motor 1) 0-180°(Motor 2) |
0-360°(Motor 1) 0-180°(Motor 2) |
|
| 最佳測量角度范圍 Optimal measurable angles |
15-80°(incident angle) (for prism 1.845) |
15-80°(incident angle) (for prism 1.845) |
15-80°(incident angle) (for prism 1.845) |
|
| 轉臺防撞系統 Anti-collision system |
兩個行程開關 AZ-721, detector motor 超出 0-180°范圍時關閉電機2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. | 兩個行程開關 AZ-721, detector motor 超出 0-180°范圍時關閉電機2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. | 兩個行程開關 AZ-721, detector motor 超出 0-180°范圍時關閉電機2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. | |
| 角度校準 Angular calibration |
半自動 Semi-automatic |
半自動 Semi-automatic |
半自動 Semi-automatic |
|
| 載物臺 Stage |
平臺控制類型 Motion Type |
手動 Manual |
手動 Manual |
手動 Manual |
| 移動平臺 Translation stage |
XYZ 移動平臺 | XYZ 移動平臺 | XYZ 移動平臺 | |
| 檢測器 Detector |
光檢測器 Photo detector |
光電二極管 Photo-diode detector |
光電二極管 Photo-diode detector |
光電二極管+CCD相機 A photo-diode detector, and a CCD camera |
| 流通系統 Flow Cell System |
進樣系統 Injection system |
蠕動泵 Peristaltic Pump |
蠕動泵 Peristaltic Pump |
蠕動泵 Peristaltic Pump |
| 型號 Type | BT100-2J | BT100-2J | BT100-2J | |
| 通道數 Pump channels |
2 | 2 | 2 or 4(Optional) | |
| 進樣控制 Injection Control |
自動和手動均可 Both Automatic and Manual |
自動和手動均可 Both Automatic and Manual |
自動和手動均可 Both Automatic and Manual |
|
| 進樣體積 Injection Volume |
20-750 μl | 20-750 μl | 20-750 μl | |
| 流通池容量 Flow Cell Capacity |
20-100 μl | 20-100 μl | 20-100 μl | |
| 流通通道數量 Flow Cell Number |
單通道 Single Channel |
單通道 Single Channel |
1-4通道(可選) 1-4 Channels (Optional) |
|
| 基本參數 Basic Parameters |
設備尺寸 Instrument Size |
1120 mm*560 mm*560 mm | 1120 mm*560 mm*500 mm | 1100 mm*560 mm*500 mm |
| 設備重量 Instrument Weight |
約120 kg | 約120 kg | 約120 kg | |
| 折射率靈敏度 Sensitivity |
10-5-10-6 RIU Depends on the sensor chips |
10-6 RIU Depends on the sensor chips |
10-6 RIU Depends on the sensor chips |
|
| 配件 Accessories |
棱鏡 Prism |
折射率/Refractive index, 1.745或1.845 可調換/Exchangeable |
折射率/Refractive index, 1.745或1.845 可調換/Exchangeable |
折射率/Refractive index, 1.745或1.845 可調換/Exchangeable |
| 流通池 Flow cell |
Quartz and PDMS | Quartz and PDMS | Quartz and PDMS | |
| 管 Tubes |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
|
| 匹配油 Matching Oil |
1.7000±0.0002 Cargille Labs |
1.7000±0.0002 Cargille Labs |
1.7000±0.0002 Cargille Labs |
|
| 樣品芯片 Sample Chips |
Sensor chips with 50 nm Au 可定制/Customizable |
Sensor chips with 50 nm Au 可定制/Customizable |
Sensor chips with 50 nm Au 可定制/Customizable |
|
| 其他 Others |
電源 Power Supply |
220V/50 HZ AC | 220V/50 HZ AC | 220V/50 HZ AC |
| 軟件 Software |
Sensby SPR | Sensby SPR | Sensby SPR | |
| 操作系統 System |
Windows 7, 10 | Windows 7, 10 | Windows 7, 10 | |
| 蛋白質組學研究(Proteomics) | 免疫測定法(Immunoassay) |
| 新藥研發(Drug Discovery) | 疫苗開發(Vaccine Development) |
| 癌癥研究(Cancer Research) | 瞬時結合(Transient Binding) |
| 信號傳遞 (Cell Signaling ) | 配體垂釣(Ligand Fishing) |
| 多分子復合物的結構和組裝( Multi-molecular Complexes) | 結合特異性(Binding Specificity) |
| 分子識別(Molecular Recognition) | 結構與功能的關系(Structure-function Relationship) |
| 免疫調節(Immune Regulation) | 酶反應(Enzyme Reaction) |
如圖所示,SPR 分子相互作用儀器的原理是波長 632.8 nm 的激光形成的 p 偏振光,到達棱鏡后激發金屬表面的表面等離子體發生共振。通過改變入射光角度 θ,實時檢測反射光強度的變化 R,根據反射光強度變化 ΔR 或共振角度的變化 Δθ,實現分子相互作用的檢測;也可以通過固定角度,測量反射光強的變化曲線獲得分子相互作用動力學常數。
| 型號/Model | SPR-T210 | SPR-I400 | |
| 檢測器 Detector |
光檢測器 Photo detector |
光電二極管 Photo-diode detector |
CCD CCD |
| 流通系統 Flow Cell System |
進樣系統 Injection system |
蠕動泵 Peristaltic Pump |
注射泵 Peristaltic Pump |
| 進樣控制 Injection Control |
半自動 Semi-Automatic |
自動 Automatic |
|
| 樣品臺 Sampling |
-- | 96孔板 自動切換 |
|
| 進樣體積 Injection Volume |
20-750 μl | 20-750 μl | |
| 流通池容量 Flow Cell Capacity |
20-100 μl | 20-100 μl | |
| 流通通道數量 Flow Cell Number |
單通道 Single Channel |
4通道 4 Channels |
|
| 基本參數 Basic Parameters |
設備尺寸 Instrument Size |
1120 mm*560 mm*500 mm | 850 mm*630 mm*611 mm |
| 設備重量 Instrument Weight |
約120 kg | 約50 kg | |
| 樣品折射率測量范圍 Measurable Sample Refractive Index |
1~1.5 | 1~1.5 | |
| 折射率靈敏度 Sensitivity |
~10-6 RIU Depends on the sensor chips |
~10-6 RIU Depends on the sensor chips |
|
| 濃度定量靈敏度 Sensitivity for Concentration Detection |
10 ng/ml | 10 ng/ml | |
| 定量范圍 Detection Range |
0.1 μg/ml – 5 mg/ml | 0.1 μg/ml – 5 mg/ml | |
| 檢測濃度范圍 Detection Range |
0.05 nM-1 mM | 0.05 nM-1 mM | |
| 檢測解離常數(KD)范圍 KD Range |
0.05 nM-0.1 mM | 0.05 nM-0.1 mM | |
| 配件 Accessories |
棱鏡 Prism |
折射率/Refractive index, 1.845 可調換/Exchangeable |
折射率/Refractive index, 1.845 可調換/Exchangeable |
| 流通池 Flow cell |
Quartz and PDMS | Quartz and PDMS | |
| 管 Tubes |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
2 Stops, Tygon R3607, ID:0.64mm; Wall:0.91mm |
|
| 匹配油 Matching Oil |
1.7000±0.0002 Cargille Labs |
1.7000±0.0002 Cargille Labs |
|
| 樣品芯片 Sample Chips |
Sensor chips with 50 nm Au 可定制/Customizable |
Sensor chips with 50 nm Au 可定制/Customizable |
|
| 其他 Others |
電源 Power Supply |
220V/50 HZ AC | 220V/50 HZ AC |
| 軟件 Software |
Sensby SPR | Sensby SPR | |
| 操作系統 System |
Windows 7, 10 | Windows 7, 10 | |